| abstract |
A method making it possible, in one embodiment, to determine the presence of an analyte in a sample of biological fluid in the presence of a substance disturbing the analysis aimed at determining the presence of said analyte. The implementation of this embodiment consists in using antibodies intended to cause the selective immunoreaction of at least the analyte or the disturbing substance, and then in carrying out an analysis aimed at determining the presence of the analyte in at least one of the immunoreactives or non-reactants. Another embodiment provides a disposable reaction device for implementing the method. The invention is applicable to the detection of a wide variety of analytes, including cholesterol in one targeted lipoprotein class in the presence of cholesterol in another class, to targeted isozymes of enzymes such as creatine kinase, lactate dehydrogenase, amylase, and alkaline or acid phosphatases in the presence of other isozymes, as well as targeted immunoglobulins in the presence of non-targeted immunoglobulins. |