http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0525836-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5128cfc84a0fb189fceb3cd240c44493 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-07 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-00 |
| filingDate | 1987-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f01f6dfc0ac2603afe654c6fafbf65fd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d20ba7a745a48cadd8eb4751af4b9c15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d721708f216af6840c0555ccb57db825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86608b63dd81dbd68b71936a52eae9fa |
| publicationDate | 1993-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0525836-A2 |
| titleOfInvention | Assay method using novel NAD synthetase |
| abstract | A method of assaying a specimen for ATP, deamide-NAD, ammonia including ammonia in the form of an ammonium ion or a component of a reaction system in which ATP, deamide-NAD or ammonia including ammonia in the form of an ammonium ion is generated or consumed, which method comprises:n (i) incubating the specimen with a NAD synthetase having the following properties:n (1) Action:n at least catalyses in the presence of Mg ++ on Mn ++ ion the reaction (a):n nand does not catalyse in the presence of Mg ion the reaction (b):n (2) Substrate specificity:n utilises ammonia, including ammonia in the form of an ammonium ion, as a substrate but does not utilise glutamine or asparagine as a substrate; in the present of Mg or Mn ions and the two of ATP, deamide-NAD and ammonia which may be in the form of an ammonium ion which are not being assayed for or which are not generated or consumed by the said reaction system, to generate thereby NAD; n (ii) subjecting the thus-generated NAD to a coenzyme cycling reaction comprising reducing said generated NAD to reduced NAD and oxidizing the thus-generated reduced NAD to NAD; and (iii) measuring a compound which is consumed or generated in said cycling reaction. |
| priorityDate | 1986-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 542.