| abstract |
A new genetic engineering process for the production of S - (+) - 2,2-dimethylcyclopropanecarboxamide is described. For this purpose, a new DNA was isolated from a microorganism, which codes for a stereospecific hydrolase. This DNA is then ligated into an expression vector, producing a hybrid plasmid which is transformed into microorganisms. These microorganisms are then able to biotransform the R - (-) - isomer to R - (-) - 2,2-dimethylcyclopropanecarboxylic acid in the racemic R, S- (±) -2,2-dimethylcyclopropanecarboxamide, with optically active S- ( +) - 2,2-Dimethylcyclopropanecarboxamide is obtained. |