http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0521111-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0fc19d8d6ba9ad5c1fa5770341472b56 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-706 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1991-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc6c5804560c73af8e7458753c38fb37 |
| publicationDate | 1993-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0521111-A1 |
| titleOfInvention | Polynucleotide capture assay employing in vitro amplification |
| abstract | An analyte polynucleotide strand having an analyte sequence is detected in a sample containing polynucleotides by bringing the analyte polynucleotide into contact with a capture probe under hybridization conditions, the capture probe having a first binding partner specific for a second solid phase binding partner. The resulting duplex is then immobilized by specific binding between the binding partners, and the unbound polynucleotides are separated from the bound elements. In some cases, the analyte polynucleotide is moved from the solid phase and then amplified by PCR. The PCR primers each have a polynucleotide region capable of hybridizing to a region of the analyte polynucleotide, and at least one of the primers additionally has an additional binding partner capable of binding to a solid phase binding partner. The amplified product is then separated from the reaction mixture by specific binding between the binding partners, and the amplified product is detected. |
| priorityDate | 1990-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 271.