http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0517704-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7a5389a45a98fa3b41048c16b073b9e3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-708 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
| filingDate | 1991-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1996-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87414809fffe34a5992ba7b062469453 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_358e9fb71e46a7b611c2a2768f59e292 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_380017b278d29e317bec1bac6cf6d735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b622a7fd5b7d885f1a8acba5e295f1dd |
| publicationDate | 1996-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0517704-B1 |
| titleOfInvention | Primers and process for detecting human papillomavirus genotypes by pcr |
| abstract | PCT No. PCT/NL91/00009 Sec. 371 Date Jul. 16, 1992 Sec. 102(e) Date Jul. 16, 1992 PCT Filed Jan. 18, 1991 PCT Pub. No. WO91/10675 PCT Pub. Date Jul. 25, 1991.The invention relates to primers and a method of detecting human papilloma virus (HPV) genotypes by means of the Polymerase Chain Reaction (PCR). The invention provides such primers and such PCR conditions that in principle any genital HPV genotype is detected. The general primers according to the invention consist of an oligonucleotide which comprises at the 3' end one of the sequences of SEQ ID NO: 3-6, or a sequence which is homologous to one of these four sequences as to at least 75%. In order to obtain amplification even in the case of a limited number of mismatches, in the PCR a relatively low annealing temperature of 30 DEG -50 DEG C. and a relatively high Mg+2 concentration of 2-10 mM are used. The invention enables a sensitive and reliable preselection of samples to be examined, such as cervical smears. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2913407-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2267155-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2272988-A2 |
| priorityDate | 1990-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.