| abstract |
A recombinant protein immobilized on a substrate is obtained by expression of a fusion protein gene in gram-negative bacteria which encodes at least one non-lytic hydrophobic domain of the protein having a membrane integrating effect, as well as the protein itself, and by the expression of a gene encoding a lytic membrane protein from bacteriophages or the lytic toxin-releasing gene or lytic partial sequences thereof, the recombinant protein immobilized on a substrate being recovered in the culture broth. The recombinant protein is incorporated in a fixed or complex manner into the cell wall of gram-negative bacteria, by means of a target sequence. The invention also relates to a recombinant DNA for producing the protein, its production method and the use of the recombinant protein immobilized on a substrate for the purposes of immunization and vaccination. |