| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-0059
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B60-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00495
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B60-14 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
| filingDate |
1986-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2001-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6f7d715bcf1afae0d498e8f15e0b2f1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70d9bc51cb2e69e4b6dc803a4df63e7d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe31b955f5c97e668365b219ac0d5338
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69fa89497d9a196e20dc255b02dac9f1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6e13536e347e2b4cbd02483e583adef
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_deff7e6e3a7b95c99a4950cdb62368f1 |
| publicationDate |
2001-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0502589-B1 |
| titleOfInvention |
Kit for use in amplifying and detecting nucleic acid sequences |
| abstract |
The present invention provides a process for detecting the presence or absence of at least one specific nucleic acid sequence in a sample or distinguishing between two different nucleic acid sequences in said sample, wherein each nucleic acid sequence to be detected consists of two separate strands, which process comprises amplifying the specific sequence or sequences (if present) by (a) treating the sample with one oligonucleotide primer for each of the two strands of each different specific nucleic acid sequence being detected under hybridizing conditions such that for each strand of each different sequence being detected an extension product of each primer is synthesized which is complementary to each nucleic acid strand, wherein said primers are selected so as to be substantially complementary to each strand of each specific sequence such that the extension product synthesized from one primer, when it is separated from its complement, serves as a template for synthesis of an extension product of the other primer; (b) treating the product of step (a) under denaturing conditions to separate the primer extension products from their templates; (c) treating the product of step (b) with oligonucleotide primers such that a primer extension product is synthesized using each of the single strands produced in step (b) as a template; and detecting the thus amplified sequence or sequences (if present). |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9598723-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8709814-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8883455-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9150908-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8691969-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8569020-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8569019-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8221682-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8546110-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8192992-B2 |
| priorityDate |
1985-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |