Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7645137dbd71c326048c3b740eb130ba |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6439 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-101666 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-0205 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-1456 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-02 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-49 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-14 |
filingDate |
1991-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75ae58887fb853d4ab5164d1d0d716d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2bd37e3d7d34c3f17fc53700a61df6f2 |
publicationDate |
1992-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-0499693-A2 |
titleOfInvention |
Method of differentiating erythroblasts from other cells by flow cytometry |
abstract |
A method which comprises using a specified dye taken up by erythrocytic nucleated cells so that their nuclei are stained and can be readily differentiated from other cells by measurement with a flow cytometer is disclosed. n The method for differentiating erythroblasts from other cells relies basically upon flow cytometry by two-step staining that uses a first fluid that is an acidic hypotensive fluorescent dye solution and a second fluid that is a solution that changes the osmorality and pH of the first fluid. The first fluid further contains a fluorescent dye that diffuses into erythroblasts to specifically stain their nuclei, and a group of erythroblasts is separated from other cell groups on the two-dimensional plot constructed with the flow cytometer, whereby the results of erythroblast differentiation are computed. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6664110-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10928405-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9709556-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1004880-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9063127-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1004880-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02088669-A1 |
priorityDate |
1991-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |