http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0499621-B1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6ac661be0b9a884290451af2b1432123 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0089 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
filingDate | 1990-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1996-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8ba8428929186b0acb2373e126c8944 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_daa1d4aade6fc9d6bac1501f76347373 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9354e34aa26eeb2a145293891af5fab2 |
publicationDate | 1996-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0499621-B1 |
titleOfInvention | Improved method for purification of recombinant copper/zinc (cu-zn) superoxide dismutase from bacteria or eucaryotic cells |
abstract | Disclosed is a method of increasing the yield of recovered solutions having an increased concentration of b isoform of an enzymatically-active polypeptide analog of Cu-Zn superoxide dismutase from a composition which comprises cells containing a, b and c isoforms of the polypeptide analog comprising treating the composition to separate soluble proteins from whole cells, cellular debris and insoluble proteins to obtain a solution containing such soluble proteins, including the a, b and c isoforms; treating the resulting solution with soluble proteins to produce three separate solutions, each of which has an increased concentration of one of either of the a, b or c isoform; recovering the separate solution with an increased concentration of the b isoform; combining the separate solution with an increased concentration of the a isoform with the separate solution with an increased concentration of the c isoform; treating the resulting combined solution to produce a solution with an increased concentration of the b isoform and recovering the then-resulting solution with an increased concentration of the b isoform. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5871729-A |
priorityDate | 1989-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 266.