http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0497798-B1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3e1018b924ad1e238642eaad9d2172a7 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01023 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2471 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-38 |
filingDate | 1990-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1995-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_76be954c9db6009ce36502833fabe6e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60b7947dca2944c83301ac34b8cedcdf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4be9d92da464e41ec5130d85774debd5 |
publicationDate | 1995-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0497798-B1 |
titleOfInvention | Process for conducting site-directed mutagenesis |
abstract | The present invention relates to an approach for conducting site-directed mutagenesis using double-stranded DNA templates. The approach involves the development of a method for generating structures capable of directing full-length complementary-strand synthesis from double-stranded plasmid DNA. These structures are formed following heat denaturation and cooling of linearized plasmid DNA molecules in the presence of what is referred to as a "closing oligonucleotide". A "closing oligonucleotide" is a single-stranded oligonucleotide consisting of a sequence complementary to either or both free ends of one of the two plasmid DNA strands. The "closing oligonucleotide" therefore functions as an agent for recircularization of a DNA strand and generation of a primer-circular template structure suitable for polymerase-dependent full-length complementary-strand synthesis and ligation into a covalently-closed heteroduplex DNA molecule. When combined with a mutagenic oligonucleotide and uracil-substituted DNA templates, this approach allows site-directed mutagenesis to be performed directly on double-stranded DNA with a mutant formation efficiency of about 50%, a level amenable to rapid screening by DNA sequencing. |
priorityDate | 1989-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 728.