| abstract |
The process for treating colostrum consists in bringing the colostrum, preferably skimmed, into contact with a cation exchanger, for example a resin with sulfonic functions, in subjecting the displaced part of the exchanger by elution to adsorption chromatography on hydroxyapatite and recovering by elution the fraction retained on the hydroxyapatite, by washing with a phosphate buffer at a concentration of at most 500 mM, preferably about 100 mM. The retained fraction, called the active fraction, contains more than half of the growth factors of the initial colostrum and is practically devoid of proteases. It is used as an additive in cell culture media, in particular as a substitute for fetal calf serum. |