http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0470230-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7da5c8e8c3e8261efe7e698c7198681b |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
filingDate | 1991-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1ec56fe85aa6030c53e20b82238150c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c713fb0ab5d5cccf24a174d945b2a52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_269b1b10c5e815aab2bd964a1d8c4426 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0876bf3eec09d7a45efa5ece0207e1aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7f50bfcd7057c46847d87b0f1ccb509 |
publicationDate | 1992-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0470230-A1 |
titleOfInvention | Method for obtaining and purifying the carboxyl-terminal fragment of the dna polymerase i of streptococcus pneunomiae |
abstract | Disclosed is a method for subcloning a fragment of the gene po1A of S.pneumoniae into an expression vector E.coli. With the disclosed method, it has been possible to obtain the recombinant plasmid pSM10. Said plasmid codes for a polypeptide of 70.6 kDa which has the polymerase activity free of exonuclease activity of the enzyme DNA polymerase-exonuclease of S.pneumoniae. The polypeptide has been hyperexpressed and purified from E.coli. The yield of active enzyme obtained corresponds approximately to 7 % of the cellular proteinic content. 0.73 mg of pure protein has been obtained from 500 ml of culture, with a specific enzyme activity of 13537 units of DNA polimerase per mg of protein. |
priorityDate | 1990-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 124.