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filingDate 1991-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7855cbc8c8c71452a9e1106db47d5949
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publicationDate 1991-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0462798-A2
titleOfInvention Release and isolation of O-glycans
abstract The invention relates to a method for the release of O-glycan from a glycoconjugate and to isolation of such glycan. n The invention provides a method which includes subjecting a glycoconjugate to the influence of a hydrazine reagent, said glycoconjugate being substantially salt-free and substantially anhydrous and said hydrazine reagent being substantially anhydrous, and controlling conditions under which the glycoconjugate is subjected to the influence of the hydrazine reagent so as to cause release of O-glycan from the glycoconjugate in such a way as to allow subsequent recovery of O-glycan in substantially unreduced and intact form. n Released glycan may be isolated in accordance with the invention by use of a chromatographic material and, if required, performing an N-acetylation reaction and converting any acetohydrazone derivative to unreduced O-glycan.
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