http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0458876-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
| filingDate | 1990-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1993-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1993-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0458876-B1 |
| titleOfInvention | Method of separating oligonucleotides from a mixture |
| abstract | A method of purifying full length synthetic target oligonucleotides from a mixture of oligonucleotides, particularly from a mixture containing truncated or failed sequences. The method involves attaching a short nucleotide sequence complementary to the 5' end of a target oligonucleotide to a solid support. The complementarity between the most 5' nucleotides of the target oligonucleotide and the bound oligonucleotide results in hybridization which serves to retain the target oligonucleotide. Truncated or failed sequences lacking 5' sequences complementary to the attached oligonucleotide, fail to hybridize and therefore are not retained. The method makes it possible to purify gram quantities of synthetic deoxyribonucleic acids or ribonucleic acids and sequences which have modifications, such as on the phosphate backbone. The support-bound nucleotide sequences are stable under conditions of purification and therefore can be reused. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0155451-A1 |
| priorityDate | 1989-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 115.