| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41686a979f961bce6a9855b4b409a5b8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-904
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-97
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-805 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54366
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54386
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 |
| filingDate |
1991-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dae442296a861ff382bd8038fbca6ac
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b4300a666c88938f5d29d2714889395
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca2de16de9ec409c91d849752b8ac345 |
| publicationDate |
1991-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0458231-A1 |
| titleOfInvention |
Substrate composition, immunoassay and urea determination employing immobilized urease |
| abstract |
A method for enzyme immunoassay of a ligand includes urease as a label. A bound fraction of ligand and antiligand conjugated to urease is formed on a solid support. The urease component of the bound fraction is contacted with a substrate composition for urease which includes a compound converted to ammonia by the urease, a tetrazolium salt and a pH dependent reducing agent which reduces the tetrazolium salt when the pH of the assay medium has been raised by the ammonia. The tetrazolium salt is reduced to a colored insoluble formazan which precipitates as a detectable spot on the support. The invention includes the substrate composition and a kit of materials for performing the assay. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2853077-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5314804-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8206944-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1845375-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1845375-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8198073-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9319200-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0605828-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9928751-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004088320-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5420016-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8460863-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0614987-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0919811-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6723524-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02052263-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-100347548-C
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004050910-A1 |
| priorityDate |
1990-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |