| abstract |
Solvent systems have been developed which are used to increase the solubility of 3,3'5,5'-tetramethylbenzidine (TMB), and to produce a permanent color record of ELISAs results using HRP (peroxidase). horseradish). In the improved solvent system containing povidone, 1-ethenyl-2-pyrrolidone polymers, TMB can be dissolved in a very small amount of solvent, up to a concentration between 100 and 200 mM, which can then be mixed directly in an aqueous buffer without precipitating the TMB. In the solvent system for producing a permanent recording, alginic acid (AA), methyl vinyl ether / maleic anhydride copolymer (MVE / MAC), dextran sulfate (SD) and / are added. or carrageenan in aqueous buffer solution, with or without povidone, to form a colored precipitate with the reaction product. |