http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0450060-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bdfe8ab7dd5d8a1785af1d164aa81fe3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_058e0be30e00d53858c67167df60aed5 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N35-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 1990-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfe4ff532ebe440361061e64ba6a51e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6085d80338efbcd4ba5e433d6059f05e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07497c2709ed85c4835c31e2f9e0155b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f739363f38785ccfd4631b55d4f166c |
publicationDate | 1991-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0450060-A1 |
titleOfInvention | Dna sequencing |
abstract | Instrument and method for determining the nucleotide sequence found in a DNA molecule, without the use of a gel electrophoresis step. The method employs an unknown primed single-stranded DNA sequence, which is immobilized or trapped inside a chamber using a polymerase, so that the complementary DNA formed sequentially can be checked each time addition of a blocked nucleotide, by measuring the presence of a harmless marker on specified deoxyribonucleotides. The invention also relates to a method for determining the unknown DNA nucleotide sequence using blocked deoxynucleotides. The blocked dNTP (deoxyribonucleotide-triphosphate) has a harmless marker, so its identity can be easily determined. The instrument and methods of the invention make it possible to obtain an accurate and rapid determination of a DNA nucleotide sequence without gel electrophoresis. |
priorityDate | 1989-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 316.