abstract |
The present invention provides industrially useful mutant luciferase. The luciferase of the invention is produced by culturing a microorganism belonging to the genus Escherichia which harbors a recombinant DNA containing the mutant luciferase gene of a firefly. Mutant luciferase can produce light of wavelengths which cannot be produced by wild type luciferase and may be used to measure accurately the amount of ATP in a solution coloured red (e.g., blood), orange, or green in which wild-type luciferase has not provided reliable results. |