| abstract |
The invention relates to the production and use of gene banks of human antibodies (AK). Starting from a mixture of human B lymphocytes, their mRNA is translated into cDNA using oligo-dT primers. The AK-specific cDNA is then amplified by means of polymerase chain reaction (PCR, polymerase chain reaction) using suitable oligonucleotide primer sequences. By expressing this amplified AK-specific cDNA in a bacterial expression vector, for example the vector pFMT described below, in E. coli, a human antibody library with a comprehensive repertoire for screening ("screening") with selected antigens is thus available in vitro Available. |