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filingDate 1990-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1991-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0431950-A1
titleOfInvention Assaying interferons
abstract Antiserum which can recognise all sub-types of human leukocyte interferon is prepared from the blood of an animal immunised with partially purified human leukocyte interferon obtained from a culture of human leukocyte stimulated with Sendai virus. n The antiserum is added to a column whereon concentrated culture broth of human leukocyte has been immobilised to absorb impurities, the effluent is added to a column whereon partially purified human leukocyte interferon has been immobilised to absorb anti-human leukocyte interferon antibody and then the antibody is eluted from the column. n The antibody thus obtained recognises all sub-types of human leukocyte interferon and can be separated by a chromatography of each monoclonal antibody which recognises a single sub-type. n Employing these antibodies, the sub-types of human leukocyte interferon or their antibodies in a sample can be analysed or assayed.
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