http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0429467-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69c91bbf0395db010b4806f50d808c4e |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-68 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 |
filingDate | 1989-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a79673a4035906e99b91c4f4e0ba08d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d421f9b5668cd46487fdeccff798be4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83bae0fb684bc1c7c28cd98e6ee99972 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12a9a2f5b89b36ebd51769e8e5a3698c |
publicationDate | 1991-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0429467-A1 |
titleOfInvention | Positive selection system for stabilisation of expression vectors |
abstract | The invention relates to a method for the construction of plasmids ensuring the insertion of a nucleotide sequence into a chromosome and the simple separation of clones containing the transformed chromosome using known genetic manipulation methods, comprising inserting a copy of a chromosomal segment corresponding to a chromosomal segment of the host cell between the transcriptional regulatory sequences, capable of expressing a foreign gene in a plasmid containing transcriptional regulatory sequences capable of expressing a foreign gene as well as a foreign gene in a plasmid containing transcriptional regulatory sequences capable of expressing a foreign gene, and a resistance gene (R2) expressed independently of said regulatory sequences; a resistance gene without its own promoter (R1) different from (R2) is then inserted downstream, then, finally, a sequence containing at least one transcription terminator in the inserted copy of the chromosomal segment. |
priorityDate | 1988-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 164.