| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5022999f224cf5d389c524abf0213be7 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P17-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-127
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-46
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P17-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K8-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K8-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K8-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-127
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K8-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61Q17-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
1989-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7857dad6e96bdce7c29991ce7703f513 |
| publicationDate |
1991-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0423214-A1 |
| titleOfInvention |
CLEANING AND ADMINISTRATION OF DNA REPAIR ENZYMES. |
| abstract |
Methods for the purification of DNA repair enzymes in which an aqueous solution of an impure DNA repair enzyme is applied to a molecular sieve column having an exclusion limit which delays the enzyme DNA repair, but not contaminants larger than this enzyme. The DNA repair enzyme in a state of increased purity is isocratically eluted from the molecular sieve column in an elution buffer system and applied directly to a DNA affinity column in the same buffer system without intermediate operation of dialysis, ultrafiltration or other procedures. The DNA repair enzyme is eluted from the column with DNA affinity, in particular by means of a salt gradient. The method is quick, inexpensive and simple to perform, and has been found to give a homogeneous end product. According to other aspects of the invention, the purified DNA repair enzymes are encapsulated in liposomes and administered to living cells in situ. It was found that this form of administration was not toxic to cells and resulted in increased incision of damaged DNA, enhanced DNA repair synthesis and increased cell survival time after exposure to ultraviolet light. In some preferred embodiments, the DNA repair enzymes are administered in pH sensitive liposomes. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012168401-A1 |
| priorityDate |
1988-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |