| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_61f88efcda59d7062a4252ecf6886f58 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
| filingDate |
1989-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
1992-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0418320-A4 |
| titleOfInvention |
Sequencing dna; a modification of the polymerase chain reaction |
| abstract |
A new process has been developed which facilitates separation of DNA strands following the polymerase chain reaction. The process comprises 1) reacting a double stranded DNA with a biotinylated primer and a non-biotinylated primer to provide a double-stranded DNA wherein one strand has biotin bound to the extension primer; 2) reacting the product of step 1 with a support that will bind the biotin moiety; 3) denaturing the DNA; and 4) separating the amplified single-stranded DNA lacking the biotin moiety from the mixture containing the amplified DNA having biotin bound thereto. |
| priorityDate |
1988-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |