| abstract |
A method of fractionating plasma protein which comprises treating a plasma protein-containing material in the following sequence of steps, provided that steps (ii) through (v) may be performed in an optional order:n (i) Freeze-thaw treatment (ii) Treatment at 5 to 10% ethanol concentration (iii) Treatment with an anion exchanger (iv) Affinity chromatography with immobilized lysine (v) Affinity chromatography with immobilized heparin (vi) Treatment at 18 to 30% ethanol concentration (vii) Treatment at 35 to 45% ethanol concentration |