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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-703
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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
filingDate 1990-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1994-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb4ffd14a15fc9743d8a484bda63eb55
publicationDate 1994-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0393743-B1
titleOfInvention Diagnostic kit, primer composition and their use for replication or detection of nucleic acids
abstract Amplification, replication or detection of a predetermined target nucleic acid can be carried out using a unique primer composition. This composition comprises an aqueous mixture of a first oligonucleotide primer which is substantially complementary to a first specific nucleic acid sequence of the target, but which is suspected of having one or more mismatches with the target at or near its 3 min end. Also included in the composition is one or more additional primers which are complementary to a nucleic acid sequence of the target. This sequence is either: (i) inclusive of only a portion of the first specific nucleic acid sequence, (ii) immediately adjacent to the first specific nucleic acid sequence, or (iii) removed from the first specific nucleic acid sequence by one or more bases, but which additional primer is capable of forming a primer extension product complementary to the first specific nucleic acid sequence. These composition components can be supplied as part of a diagnostic test kit which can include other reagents if desired.
priorityDate 1989-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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