| abstract |
It comprises: (a) adding an inorganic electrolyte to the broth; (b) incorporating an inorganic coagulant with stirring and at a pH of 5-8 in order to start the flocculation; (c) adding, once the flocculation has started, an organic polyelectrolyte at a pH of 6-8 and with stirring; (d) separating the flocculi by means of rolling sieves or simple filtration, followed by decantation if the flocculation has been done in the presence of an organic solvent; and, optionally, concentrating the liquid by reverse osmosis or evaporation. n The process is applicable in the clarification of culture broths allowing a micelle separation simultaneous to deproteinization. Likewise, the colloidal material or material in suspension is eliminated along with dissolved materials which make the purification process difficult. |