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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
filingDate 1989-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fbe4f4b4f431bded19346e414d4526d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a57db794792df2007e25d68602d95d64
publicationDate 1990-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0379559-A1
titleOfInvention METHOD AND MEANS FOR DETECTING NUCLEIC ACID SEQUENCES.
abstract A method of detecting a target sequence of a nucleic acid may include an amplification step and a detection step. For amplification, a plurality of pairs of nucleic acid amplification probes are used, the elements of each pair of amplification probes being complementary and at least one element of hybridization of each pair of amplification probes being also complementary to a given part of the target nucleic acid sequence, which serves as a model. The hybridization elements of each pair of amplification probes hybridize contiguously with the target sequence, at positions sufficiently close together that one can join the probes and form an amplification product. Once the amplification probes are joined, the completed amplification product can be separated by denaturation and the process repeated until a sufficient quantity of the target nucleic acid sequence is obtained to generate a measurable signal during the selected analysis. The correctly assembled amplification product serves as a model, in the same way as the target nucleic acid sequence served as a model during the amplification step.
priorityDate 1988-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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