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filingDate 1988-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1990-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0375700-A1
titleOfInvention Determination of ambient concentration of several analytes
abstract A method of determining ambient concentrations of a plurality of analytes in a V-liter liquid sample, comprising loading a plurality of various binding agents, each capable of reversibly binding an analyte which is or may be present in the liquid sample and is specific for that analyte, compared to the other constituents of the liquid sample, on a support means and at a plurality of locations spaced such that each location has an amount of not more than 0.1 V / K, preferably less than 0.01 V / K mole of a single binding agent, where K liters / mole represents the equilibrium constant of the binding agent intended for the analyte. The method then consists in bringing the load support means into contact with the liquid sample to be analyzed, so that each of the spaced locations is brought into contact, in the same operation, with the liquid sample, the quantity of liquid used in the sample being such that only an insignificant proportion of any analyte present in the liquid sample becomes bound to the specific binding agent thereof. The method further comprises measuring a parameter representative of the fractional occupation of the binding agents by the analytes, at spaced locations, according to a competitive or non-competitive assay technique, employing a site recognition reagent for each binding agent capable of recognizing either the unfilled binding sites, or the filled binding sites, on the binding agent, said site recognition reagent being marked by means of a marker making it possible to measure the quantity of said reagent located in the particular location. Also described are a device and a kit for use in the process.
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