| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_84d9adaa7e7ce01ce8020f5c721aba10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
| filingDate |
1989-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1995-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2aa5c68583c0a5654b42f4584e31e94
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f597d74adddf0ed6fa9918dd9cf6a845
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b848bec4d0a16fe8cbe78850f7ef311
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d1d6090dc9870c55a2ccf7516d24934 |
| publicationDate |
1995-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0373962-B1 |
| titleOfInvention |
Isolating thermostable enzymes |
| abstract |
A thermostable enzyme free from unwanted contaminants is isolated by: (a) providing a mesophilic host cell engineered to express a gene encoding a heterologous thermostable enzyme; (b) culturing the mesophilic host cell and producing the thermostable enzyme in a mixture comprising unwanted contaminants; and (c) purifying the thermostable enzyme by a method that includes heating the enzyme and the unwanted contaminants to a temperature sufficient to inactivate the unwanted contaminants but not sufficient to inactivate the thermostable enzyme. A method of assaying for nucleic acid ligase is described which includes forming an adenyl adduct with the ligase and detecting the adduct. The latter method is used to screen a library of transformants for the ability to express the nucleic acid ligase. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102021124566-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11155840-B2 |
| priorityDate |
1988-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |