| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a5b2445a22755a0a4aa58739889b17ea |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-14143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-036
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-705 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21069
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43563
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-1232
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6459
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-72
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
| filingDate |
1989-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_996cdb302de3a6cca01e4bd3718f7bbc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c370a20365c553bbac940889b66d549
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad1c9776c73d0af80f2875137941ae41 |
| publicationDate |
1990-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0356409-A2 |
| titleOfInvention |
A method for the production and isolation of a fusion protein in eukaryotic cells |
| abstract |
A method for the production and isolation of a fusion protein, comprising the steps:n a) constructing a recombinant vector comprising a first DNA sequence coding for a desired peptide or protein and operatively linked to a second DNA sequence coding for an amino acid sequence capable of selective binding to a carrier material, said first or second DNA sequence being preceded by and being operatively linked to a third DNA sequence coding for a eucaryotic signal peptide; b) transforming a compatible eucaryotic host with the recombinant vector resulting from step a) such that the combined DNA sequences coding for said fusion protein can be expressed and secreted by said host; c) culturing said host to produce said fusion protein; d) isolating the fusion protein resulting from step c) by adsorption to a carrier material; and e) desorbing said fusion protein from said carrier or removing by cleavage said peptide or protein from said amino acid sequence adsorbed on said carrier; and f) recovering said fusion protein or said peptide or protein; nrecombinant nucleotide sequence; nrecombinant vector containing such nucleotide sequence; na process for preparing polyclonal antibodies using a fusion protein; and nthe use of the fusion protein as a vaccine or for the manufacture of monoclonal antibodies. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9404688-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6693181-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6191254-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5856187-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5643756-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5846540-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5952474-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5733548-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9405780-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7390873-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0788508-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9404688-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0788508-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9953059-A1 |
| priorityDate |
1988-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |