http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0353218-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P7-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12S3-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P7-02 |
| filingDate | 1989-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1992-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1992-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0353218-B1 |
| titleOfInvention | Method of producing lysplasminogen |
| abstract | For the production of Lys-plasminogen, with a specific activity of at least 17.5 caseinolytic units/mg of protein and at least 50 mu mol/g protein nitrogen, and an electrophoretic purity of at least 90%, entailing plasminogen being adsorbed from plasma, from a plasminogen-containing fraction or from a tissue culture for the purpose of purification on immobilised lysine, eluted and obtained from the eluate with a protein precipitant, a solution of the plasminogen purified in this way is adjusted to a plasmin activity which is, for the chromogenic substrate H-D-Valyl-L-Leucyl-L-lysine p-nitroanilide dihydrochloride, in the range from 0.005 to 0.2 mu mol/ml.min, preferably 0.01 to 0.1 mu mol/ml.min, is maintained at a temperature of +1 DEG C to +20 DEG C, preferably +4 DEG C to +12 DEG C, for a period of 6 to 60 hours, preferably 15 to 50 hours, in order to achieve enzymatic proteolytic conversion of the plasminogen into Lys-plasminogen, after which the enzyme reaction is stopped and Lys-plasminogen is isolated. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AT-407706-B |
| priorityDate | 1988-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.