| abstract |
A cell-proliferative protein present in Harder's glands of mammalian animals has the following physicochemical properties; (A) the molecular weight is 14,000 to 15,000 as measured by SDS polyacrylamide electrophoresis, (B) the isoelectric point is 4.5 to 5.5, and (C) the number of residues relative to one methionine residue is 2.8 for asparagine and asparaginic acid, 1.6 for glutamine and glutamic acid, 1.0 for methionine, 2.5 for alanine, 3.5 for isoleucine, 13.3 for serine, 3.2 for leucine, 11.8 for lysine and 2.4 for valine. Harder's glands, e.g. from guinea pigs, are homogenised and the crude extract is chromatographed to isolate the protein. In a daily adult dose of 10ng to 100ng the protein in a pharmaceutical composition is used internally or externally to treat cytoclastic diseases and as a pre-treating material in skin grafting; it also is useful for making artificial skin or liver. |