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filingDate 1988-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1990-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0344287-A4
titleOfInvention Conservative whole blood sample preparation technique
abstract A method for the rapid preparation of a whole blood sample for photooptical analysis. A whole blood sample, lytic reagent system and stain are contacted in a reaction vessel with gentle, asymmetric vortex mixing. This method conserves the endogenous constituents of the sample, since the preparation of the sample occurs within a common vessel and any excess, unconsumed or unreacted, reagents and stain are not separated from the sample during preparation; thus avoiding the multiple wash steps traditionally employed. The lytic agent preferably is acidic and stromatolyzes the erythrocyte fraction of the whole blood, leaving the leukocyte sub-populations in an unlysed, near native state. The lysing can occur, before, simultaneous with, or after the staining in an elapsed time of 90 seconds or less.
priorityDate 1987-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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