Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6cfc033f059d282fcd915bc8be2ae53f |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H6-4636 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H1-023 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H1-021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
filingDate |
1989-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee7ece955d5ac47d5f366fd07be83317 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d298931821415cda4c551b43d3b063a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c2dadf063b5f774757d0ced361a2c44 |
publicationDate |
1989-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-0327970-A2 |
titleOfInvention |
Method of producing rice cybrid cells |
abstract |
A method of producing rice cybrid cells is disclosed. The method comprises the steps of irradiating X-ray to first rice protoplasts containing a first useful gene in cytoplasm to selectively destruct the nuclei thereof only; treating second rice protoplasts containing a second useful gene in the nucleus with about 10 - 30 mM of iodoacetamide, about 0.1 - 0.5 mM Rhodamine 6G or about 10 - 30 mM iodoacetage to selectively deacrtivate the cytoplasm; and fusing the treated first and second protoplasts to form cybrid cells which have only the nucleus containing the first useful gene and have only the cytoplasm containing the second useful gene. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8058505-B2 |
priorityDate |
1988-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |