| abstract |
Urea derivatives of oxazine and thiazine chromophors of the formulae:n andn wherein n is 0 to 20; M is oxygen or sulfur; R 1 , R 2 , R 3 are hydrogen or alkyl group from 1 to 10 carbon atoms; R 4 and R 5 are hydrogen, alkyl group from 1 to 10 carbon atoms, halogen or amine group; X θ is anion and Z is N = C = 0, N = C = S, carboxylic, primary or secondary amine, and when n = 0, Z may ben wherein Q is hydroxyl, amino carboxylic, sulfydryl, iso cyanato, or isothiocyanato; are formed into adducts with organic substrates and used as fluorescent labels in analytical techniques for the detection and measurement of biological and clinical compounds of interest. |