http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0311655-A1

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filingDate 1988-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43d78694a35376854a1e96169c65d7e4
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publicationDate 1989-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0311655-A1
titleOfInvention PARTIAL DIFFERENTIAL ANALYZER DEVICE USING LIGHT DISTRIBUTION TECHNIQUES.
abstract Method and device for analyzing particles allowing the differentiation of white blood cells (leukocytes) by optical and electronic measurements, without the use of dyes, on a flow of leukocytes. A hydrodynamically focused current of these leukocytes is passed in the native state, in and through a beam (20) of radiant energy in a flow cell (10), the beam being exclusively dispersed by a different sub-population leukocyte subpopulations. The scattered light strikes the light collecting means (24) disposed adjacent to the flow cell. For detection of eosinophils, the scattered light is collected at angular positions ranging from about 10 ° to 70 ° relative to the axis (26) of the beam (20). Other leukocyte subpopulations are differentiated with respect to eosinophils and with respect to themselves by optical and / or electronic measurements, calling on the Coulter principle of particle analysis in particular.
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