abstract |
Method and reagent for carrying out an ionic assay in fluids, the influence of these ions on the activity of an enzyme being measured. The ions are for example sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or else non-metallic ions including chloride, bicarbonate, protons, and substances ammonium giving rise to ammonium. The enzymes which are used can be for example a transferase, a hydrolase, an oxidoreductase, or a lyase. An essential part of the invention is a method for excluding interference due to ions by masking the interference ions with a binding agent. |