http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0308883-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b5e07888f923b7443f3b8eb913614893 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 1988-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c8fdb2b06dd3c6cfd1ae40b5505324a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1ebd01e447833d50ef18ec15ab98223 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b605272d2139406d4df9ddba292bed5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d64fde5f53e1d649b90323fc4812094c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6388b569b0a85d76c62c48f42d296519 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9261f983e02b89d6046129a740f11ab4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d33fcd8a46c2d8cd263897627e0c4dba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a0344279dae47c39f88f7056a8c5613 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ebf00a0962a54ffbe30e8a35b3087557 |
| publicationDate | 1989-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0308883-A2 |
| titleOfInvention | Process for cloning DNA sequences or for the production of gene libraries, cloning vectors, and micro-organism strains containing them |
| abstract | a)von den kohäsiven 5 -Enden eines klonierenden Vektors, der nach einer Behandlung mit Restriktionsendonuclease als lineare DNA isoliert worden ist, die Phosphatgruppen mit Phosphatase entfernt werden, zu den zur Verbindung untereinander unfähigen Fragmenten die das zu klonierende Gen enthaltenden, durch Behandeln mit Restriktionsendonuclease erzeugten, an ihren Enden Phosphatgruppen aufweisenden DNA-Fragmente zugegeben werden und das Gemisch mit Ligase ligiert wird oder b) ein kohäsive Sequenzen enthaltender rekombinanter Klonierungsvektor vom Typ Phage, Cosmid, Phasmid oder Transphamid mit Terminase geschnitten wird und die nach einer der obigen Alternativen erhaltene monomere DNA in vitro assembliert (verpackt) wird und dann die Phagen vermehrt werden. The invention relates to a method for cloning DNA sequences or for producing gene banks, in which a) from the cohesive 5 ends of a cloning vector which has been isolated as a linear DNA after treatment with restriction endonuclease, the phosphate groups are removed with phosphatase, to the fragments which are incapable of connecting to one another and which contain the gene to be cloned, by treatment with restriction endonuclease generated DNA fragments containing at their ends are added and the mixture is ligated with ligase or b) a recombinant cloning vector of the phage, cosmid, phasmid or transphamide type containing cohesive sequences is cut with terminase and the monomeric DNA obtained according to one of the above alternatives is assembled (packaged) in vitro and the phages are then multiplied. n n n The invention also relates to methods for producing cloning vectors and cloning vectors and microorganisms containing them. |
| priorityDate | 1987-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 163.