| abstract |
The present invention relates to DNA compounds and expression vectors that encode deacetoxycephalosporin C synthetase (DAOCS) and deacetylcephalosporin C synthetase (DACS) activities. The compounds can be used to conÂstruct recombinant DNA expression vectors for a wide variety of host cells, including E . coli , Penicillium , and Cephalosporium . The invention also relates to the regulatory elements of the Cephalosporium acremonium DACS/DAOCS gene. A method for selecting Penicillium transformants, including those able to synthesize cephalosporin antibiotic due to the presence of the DACS/DAOCS-encoding DNA, is also provided. This transformation system utilizes an acetamidase gene for purposes of selecting transformants. |