| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6cfc033f059d282fcd915bc8be2ae53f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate |
1988-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a7b9af03911894b86476fa0ad673bfc0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec5243385b0e099010eb790206ff4cfa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_141f1720693385602f47af2a487eda64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da007a9e00ea623df18d9e518a1242f6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e2bca669d7741acd8a67819e038a15c |
| publicationDate |
1988-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0279664-A2 |
| titleOfInvention |
A method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign gene product thereby |
| abstract |
Escherichia coli carrying a hybrid plasmid constructed by inserting a desired foreign gene into an expression vector, was cultured in a medium containing a sugar component utilizable by the E. coli as a carbon source at a concentration of 0.3% or more so that the expression of said desired foreign gene was suppressed. This E. coli was cultured in the medium in which the sugar concentration was maintained at 0.3% or more in a first process to suppress the foreign gene and to support sufficient cell growth and thereafter at less than 0.3% in a second process to release the suppression to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9106656-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0411501-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0278706-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4946790-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0397097-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0278706-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5279951-A |
| priorityDate |
1987-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |