| abstract |
Mycobacteria can be detected immunologically directly in the sputum by subjecting a liquefied sputum sample to a 2-4 minute heat surfactant treatment, spreading out the sputum sample treated in this way, incubating the smear with a labeled polyclonal or monoclonal antibody against mycobacteria in the presence of moisture and the sample one subject to suitable immunological detection method for labeling. Particularly suitable monoclonal antibodies come from hybridoma cell lines BS 100, 102, 104 and 107. |