http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0272291-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6421 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6445 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 1987-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1992-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1992-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0272291-B1 |
| titleOfInvention | Method for measuring polarized fluorescence emissions |
| abstract | Method for the measurement of polarized emissions in which background emissions are compensated for without the necessity of separating a fluorescing material from a substrate which contributes the background fluorescence. The method is particularly useful in measuring fluorescence from a suspension of cells. The method involves the measurement of polarized fluorescence emissions in a vertical and a horizontal plane at a first wavelength and at a second wavelength. The second wavelength is selected based upon the bathochromic shift of the spectrum of the fluorescence emission of the background fluorescence as compared to the fluorescence of the material being analyzed at each of the wavelengths. From these measurements a factor representing the fraction of the total intensity of fluorescence emission due to background fluorescence is determined from which the intensity of the vertically and horizontally polarized emission intensities due to background fluorescence is derived. The derived polarization emission intensities are then deducted from the vertically and horizontally polarized emission intensities obtained at the first wavelength to obtain polarized emission intensities due solely to the material being analyzed. The method of the present invention is particularly adapted for the measurement of polarized fluorescent emissions from suspensions of cells in a substrate which, which substrate contributes background fluorescence. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104089939-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104089939-B |
| priorityDate | 1986-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.