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filingDate 1987-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6c27375fb099f3b9246f2adc7d02713
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publicationDate 1988-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0260903-A2
titleOfInvention Use of a chemical blocking agent against non-specific binding of an antibody specific for terminal deoxynucleotidyl transferase in biological specimens during immunoassays
abstract A chemical agent is provided or significantly preventing or blocking non-specific staining or binding of an antibody specific for Terminal Deoxynucleotidyl Transferase (TdT) during immunofluorescent or im­munoperoxidase assay procedures. These procedures in­clude both immunofluorescent and immunohistochemical staining of samples followed by flow cytometric and/or microscopic analysis, respectively. The invention is practiced by selective use of casein introduced into the assay procedures at an appropriate interval prior to analysis using a labelled or tagged monoclonal antibody specific to a TdT epitope. The casein util­ized successfully was obtained from a large variety of sources and includes the use of a non-fat milk product.
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