abstract |
L-arginine, L-citrulline or L-ornithine is produced by constructing a recombinant DNA containing genes coding for N-acetylglutamate kinase, N-acetyl-γ-glutamylphosphate reductase and argininosuccinase of a microorganism belonging to the genus Escherichia , incorporating the recombinant DNA in a microorganism belonging to the genus Corynebacterium or Brevibacterium , culturing the microorganism in a medium, and recovering L-arginine, L-citrulline or L-ornithine accumulated in the culture broth. Further, L-arginine is produced by constructing a recombinant DNA containing these genes and a gene coding for argininosuccinate synthetase of a microorganism belonging to the genus Escherichia , incorporating the recombinant DNA in a microorganism belonging to the genus Corynebacterium or Brevibacterium , culturing the microorganism in a medium, and recovering L-arginine accumulated in the culture broth. |