| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e70c0aeade92d7571d955d3b90ea06e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-968
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-808
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-13 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-6561
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-65586
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44721
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44726
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-6512
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D471-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D405-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D405-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D405-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D405-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D405-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D405-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D407-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D487-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-84
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D471-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09B11-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-6512
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-655
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-6561
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-6558 |
| filingDate |
1987-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1995-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_376f8c9677d049d366f3303e84e0c2d2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5b68be95de1ca0896fdd078a559bf07
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e48cd303080761ad37c54e2e4676c6a4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ec234e9bc873e98023f63a8ca96bf78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_537879ad3af2ea0af758c770ee82d0be |
| publicationDate |
1995-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0252683-B1 |
| titleOfInvention |
Process and reagents for DNA sequence analysis |
| abstract |
A DNA sequencing system and method are described to detect the presence of radiant energy emitted from different excited reporter dye-labeled species (DNA fragments) following separation in time and/or space, and the identity of the species which emit radiant energy closely spaced in wavelength. Functions of the emitted energy are obtained which vary over the wavelengths of the closely spaced spectra in different senses and the functions ratioed, whereby the ratio is indicative of the identity of the DNA fragments. The emitting portion of the reporter-labeled DNA fragment is preferably one of a family of fluorescent dyes based on 9-carboxyethyl-6-hydroxy-3-oxo-3H-xanthene. These xanthene dyes are covalently attached to the DNA fragments through the carboxylic acid functionality, preferably via an amide linkage. The dyes may be protected by including an alkoxy group at the 9-position. A spacer may be inserted between the dye and the amine. The fluorescent dye preferably is attached to the DNA chain terminators and provides many advantages. Thus only DNA sequencing fragments resulting from bona fide termination events will carry a reporter. The DNA sequencing may also be labeled using the xanthene dyes which have general utility as fluorescent labels. Also acyclonucleoside triphosphates are described as being useful as chain terminators in DNA sequencing using a modification of the Sanger method. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6596257-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7291463-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6458547-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6649357-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6649356-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6416960-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7256291-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7947817-B2 |
| priorityDate |
1986-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |