abstract |
Chimeric alpha-amylases are disclosed of the formula nQ-R-L nin which Q is a N-terminal polypeptide residue of from 55 to 60 amino acid residues which is at least 75 percent homologous to the 55 N-terminal amino acid residues in the Bacillus amyloliquefaciens alpha-amylase as described in Takkinen et al., J. Biol. Chem. 258 (1983) 1007-1013, nR is a polypeptide residue of the general formulan in which X₈ comprises His or Gln, nX₉ comprises Gly or Ser, and nX₁₀ comprises Ser or Asp; and nL comprises a C-terminal polypeptide of from 390 to 400 amino acid residues which is at least 75 percent homologous to the 395 C-terminal amino acid residues in the Bacillus licheniformis 584 (ATCC 27811) alpha-amylase. |