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filingDate 1986-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad1ae4fc1e322ead34ecd96ec15a68b9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81662bc8938f6a3f2e214052845771c9
publicationDate 1987-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0245481-A1
titleOfInvention Yeast strains.
abstract The process described makes it possible to increase the rate of production of carbon dioxide, ethanol and other fermentation products such as citric acid produced by a yeast such as Saccaromyces cerevisiae during fermentation and makes it possible to decrease the production of biomass by regulating the glycolysis rate indirectly by modifying the energy balance of the cell, that is to say by reducing the intracellular ATP levels. Modifications in order to change the glycolysis rate call for regulated ATP hydrolysis in the cell or a regulated leakage of ATP from the cell. This invention describes several ways to modify the ATP level of yeast, namely: (a) introduction of ineffective metabolic cycles to increase ATP consumption; (b) introducing a modified exocellular acid phosphatase so that it becomes intracellular for the purpose of increasing intracellular ATP hydrolysis; (c) use of a drug which uncouples ATPase from the plasma membrane thereby consuming an abnormally high level of ATP; (d) use of a drug which allows the release of ATP from the cell to lower the level of intracellular ATP. This invention also relates to the regulation of genetic modifications in more detail in the description. By these means, it is possible to stop such modifications during the growth of yeast on a commercial scale, then to restore them before or during, and preferably before or at the very beginning of the phase of rising of the dough.
priorityDate 1985-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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