abstract |
A method is provided for simultaneously clarifying and stabilizing a liquid cell culture medium comprising desired product, e.g. antibiotic, protein or carbohydrate, and one or more of (i) biological cells and/or cell debris, (ii) spent nutrients, and (iii) contaminating water-soluble haze- and/or color-generating proteinaceous materials, the method enabling a reduction of further purifying operations, increased yield of product, and significant savings in time and expense, when compared to known procedures. n The method comprises contacting the cell culture medium with particulate, polymeric adsorbent having a particle size of 0.01 to 200 micrometers, optionally in combination with water-soluble polymer. |