| abstract |
Process for the preparation of an immunogenic complex containing antigens or antigenic determinants with hydrophobic domains, in which viruses, mycoplasmas, bacteria, parasites, animal cells, antigens or antigenic determinants with hydrophobic domains are mixed with one or more agents of solubilization, complexes formed between antigens or antigenic determinants and a solubilizing agent, after which the antigens or antigenic determinants are separated from the solubilizing agent in the presence of a glycoside solution, or are separated from the solubilizing agent and directly transferred to a glycoside solution containing one or more glycosides with hydrophobic and hydrophilic domains in a concentration equal to at least the critical micellular concentration, thus forming a protein complex which is isolated and purified, characterized in that lipids are added before isolation and p urification of the complex. |