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filingDate 1986-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1988-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0233937-A4
titleOfInvention FERMENTATION PROCESSES FOR THE PRODUCTION OF HEPATITIS VACCINES.
abstract A method for culturing yeast capable of expressing an exogenous gene product wherein the yeast is cultured at about 25<o>C in a medium including balanced free amino acids at a concentration between about 20 g/l and about 160 g/l. Alternatively, yeast capable of expressing an exogenous gene product (e.g. the product of a gene introduced by the application of recombinant gene technology) may be introduced into a medium including a total carbon source concentration above but maintained as close to zero as possible, and cultured at about 25<o>C. A medium for fermentation of yeast capable of expressing an exogenous gene product includes balanced free amino acids at a concentration between about 20 g/l and about 160 g/l. The culture medium is preferably saturated with oxygen at least 30% of air saturation.
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